Composite

Part:BBa_K4343068:Design

Designed by: Xiaolu Sun   Group: iGEM22_NNU-China   (2022-10-09)


pUC-HUH-EgDes5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1920
    Illegal XhoI site found at 1953
    Illegal XhoI site found at 4708
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2108
    Illegal AgeI site found at 97
    Illegal AgeI site found at 458
    Illegal AgeI site found at 3161
    Illegal AgeI site found at 3522
    Illegal AgeI site found at 4664
    Illegal AgeI site found at 5629
    Illegal AgeI site found at 6036
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 638
    Illegal BsaI site found at 3702
    Illegal BsaI site found at 4239
    Illegal BsaI site found at 4667
    Illegal BsaI site found at 6039
    Illegal BsaI.rc site found at 4661
    Illegal BsaI.rc site found at 6033
    Illegal BsaI.rc site found at 6303
    Illegal SapI.rc site found at 363
    Illegal SapI.rc site found at 3427


Design Notes

none


Source

∆-5 desaturase from Euglena gracilis

References