DNA

Part:BBa_K4342029:Design

Designed by: Samer Salman   Group: iGEM22_Austin_UTexas   (2022-10-12)


P. destructans Integration Cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1040
    Illegal EcoRI site found at 6636
    Illegal XbaI site found at 180
    Illegal XbaI site found at 3035
    Illegal XbaI site found at 6556
    Illegal SpeI site found at 6234
    Illegal PstI site found at 645
    Illegal PstI site found at 1802
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1040
    Illegal EcoRI site found at 6636
    Illegal NheI site found at 7175
    Illegal SpeI site found at 6234
    Illegal PstI site found at 645
    Illegal PstI site found at 1802
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1040
    Illegal EcoRI site found at 6636
    Illegal BglII site found at 6394
    Illegal BglII site found at 6892
    Illegal BamHI site found at 3029
    Illegal BamHI site found at 4696
    Illegal XhoI site found at 3191
    Illegal XhoI site found at 4731
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1040
    Illegal EcoRI site found at 6636
    Illegal XbaI site found at 180
    Illegal XbaI site found at 3035
    Illegal XbaI site found at 6556
    Illegal SpeI site found at 6234
    Illegal PstI site found at 645
    Illegal PstI site found at 1802
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1040
    Illegal EcoRI site found at 6636
    Illegal XbaI site found at 180
    Illegal XbaI site found at 3035
    Illegal XbaI site found at 6556
    Illegal SpeI site found at 6234
    Illegal PstI site found at 645
    Illegal PstI site found at 1802
    Illegal NgoMIV site found at 4847
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References