DNA

Part:BBa_K4342020:Design

Designed by: Adam Franco   Group: iGEM22_Austin_UTexas   (2022-10-11)


ACIAD2049 Rescue Cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1040
    Illegal EcoRI site found at 1906
    Illegal XbaI site found at 180
    Illegal XbaI site found at 1826
    Illegal SpeI site found at 1504
    Illegal PstI site found at 645
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1040
    Illegal EcoRI site found at 1906
    Illegal NheI site found at 2445
    Illegal SpeI site found at 1504
    Illegal PstI site found at 645
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1040
    Illegal EcoRI site found at 1906
    Illegal BglII site found at 1664
    Illegal BglII site found at 2162
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1040
    Illegal EcoRI site found at 1906
    Illegal XbaI site found at 180
    Illegal XbaI site found at 1826
    Illegal SpeI site found at 1504
    Illegal PstI site found at 645
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1040
    Illegal EcoRI site found at 1906
    Illegal XbaI site found at 180
    Illegal XbaI site found at 1826
    Illegal SpeI site found at 1504
    Illegal PstI site found at 645
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References