DNA

Part:BBa_K4342007:Design

Designed by: Keaton Brown   Group: iGEM22_Austin_UTexas   (2022-10-04)


cymR Repressor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 701
    Illegal XhoI site found at 736
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 852
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed to ligate to the tdk/kan cassette and the P. destructans target sequence downstream via bsaI restriction sites on 5' and 3' ends.



Source

E. Coli

References