![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4340600:Design
Designed by: Chein Yueh Liu Group: iGEM22_PuiChing_Macau (2022-09-29)
T2R4 with NSP4 peptides
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 346
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 48
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We firstly searched hormone binding proteins to target reducing the effect of hormone that stops or delays seed germination. We found that T2R4 is suitable for inhibiting ABA, however, the efficiency is not very high. Therefore, we added NSP4 secretion peptides before it to help with its efficiency.
Source
BBa_K3852004 for T2R4 BBa_K2760000 for NSP4