Composite

Part:BBa_K4335021:Design

Designed by: Hang Zhang   Group: iGEM22_UESTC-BioTech   (2022-10-10)


pCrU6.3+insert site+SpScaffold+polyT Term


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 48
    Illegal PstI site found at 121
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 48
    Illegal PstI site found at 121
    Illegal NotI site found at 453
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 48
    Illegal PstI site found at 121
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 48
    Illegal PstI site found at 121
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 551
    Illegal BsaI.rc site found at 533


Design Notes

Source

References