Reporter
Part:BBa_K4335004:Design
Designed by: Feng Zhenxing Group: iGEM22_UESTC-BioTech (2022-10-10)
mCherry
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We introduced the mCherry gene The plasmid pTX2038_piGEM22_Cas9+pCrU6_sgRNA+mCherry+APHVII (link) as its marker gene. PCrRBCS2 (link) was used as the promoter and TCrRBCS2 as the terminator to achieve the expression of the mCherry gene.
Source
mushroom coral