Reporter

Part:BBa_K4335004:Design

Designed by: Feng Zhenxing   Group: iGEM22_UESTC-BioTech   (2022-10-10)


mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We introduced the mCherry gene The plasmid pTX2038_piGEM22_Cas9+pCrU6_sgRNA+mCherry+APHVII (link) as its marker gene. PCrRBCS2 (link) was used as the promoter and TCrRBCS2 as the terminator to achieve the expression of the mCherry gene.


Source

mushroom coral

References