DNA
Part:BBa_K4329003:Design
Designed by: Yuxin He Group: iGEM22_BS_United_China (2022-09-25)
Quorum sensing suicidal system-part1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
T7 promoter is an efficient promoter for
Lac operator inhibits protein and enzyme production without the presence of IPTG
LuxR is a common system used in the quorum system. It is used to detect AHL in the environment.
T7 terminator is used to stop the transcription.
QS promoter is the original sequences from Vibrio fischeri which controls the expression of Lux IABC gene expression.
Source
BBa_Ka3882000 from BS_United_China in 2021
References
T. 0. Baldwin, J. H. Devine, R. C. Heckel, J.-W. Lin, and G. S. Shadel Department of Biochemistry and Biophysics, Texas A&M University, and Texas Agricultural Experiment Station, College Station, Texas 77843, USA