Coding
XDH

Part:BBa_K4324101:Design

Designed by: Chris Yoo   Group: iGEM22_TheKingsSchool_AU_HS   (2022-09-27)


Xylitol Dehydrogenase from S. cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 606
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 263


Design Notes

The protein sequence was codon-optimised for expression in Escherichia coli K12 through the IDT codon optimisation tool as gBlocks® Gene Fragments, and ensured that EcoRI, XbaI, SpeI, and PstI restriction sites were not accidentally present within the CDS.

Source

This original protein sequence was taken from the XYL2 gene of Saccharomyces cerevisiae (also known as Baker's yeast) (strain ATCC 204508 / S288c), which can be found on the Uniprot database.

Alternatively, the protein sequence is pasted below:

MTDLTTQEAIVLERPGKITLTNVSIPKISDPNEVIIQIKATGICGSDIHYYTHGRIANYV VESPMVLGHESSGIVALIGENVKTLKVGDRVALEPGIPDRFSPEMKEGRYNLDPNLKFAA TPPFDGTLTKYYKTMKDFVYKLPDDVSFEEGALIEPLSVAIHANKLAKIKFGARCVVFGA GPIGLLAGKVASVFGAADVVFVDLLENKLETARQFGATHIVNSGDLPHGVTVDSVIKKAI GKKGADVVFECSGAEPCVRAGIEVCKAGGTIVQVGMGQEEIQFPISIIPTKELTFQGCFR
YCQGDYSDSIELVSSRKLSLKPFITHRYSFKDAVEAFEETSHHPLNNIKTIIEGPE

References

1. https://www.uniprot.org/uniprotkb/Q07993/entry
2. https://www.genome.jp/dbget-bin/www_bget?ec:1.1.1.9