Composite

Part:BBa_K4322004:Design

Designed by: Abraham Sinfort   Group: iGEM22_Cornell   (2022-09-27)


csgAα-amajLime fusion protein with glycine-serine linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1128
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 58
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our team codon optimized the sequence of this part for the expression our composite part.


Source

Escherichia coli (strain K12)

References