Coding

Part:BBa_K4317099:Design

Designed by: Chris Song   Group: iGEM22_Seoul_Korea   (2022-10-12)


Secretion signal library


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 820
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A signal peptide library plasmid was designed so that 12 secretion signals were selected for the coding sequence of each enzyme and cut into NdeI and NcoI for use.


Source

This part is synthesized from IDTDNA.

References