Regulatory

Part:BBa_K431009:Design

Designed by: David Joseph Sexton Jr.   Group: iGEM10_Georgia_State   (2010-09-21)

glyceraldehyde 3-phosphate dehydrogenase promoter (pGAP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 245


Design Notes

The following primers were used to isolate a 0.9kb segment known to contain the pGAP regulatory activity

Forward:5'GCTCTAGGCAGCGAGCTGATCCTTTTTTGTAT

Reverse:5'GGCCACTAGTTGTGTTTTGATAGTTGTTC

Source

This part came from the genome of P. pastoris

References