Translational_Unit

Part:BBa_K4310017:Design

Designed by: Seungchan Oh   Group: iGEM22_UIncheon   (2022-10-11)


Cold inducible promoter + phiC31 recombinase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 842
    Illegal PstI site found at 1941
    Illegal PstI site found at 2800
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3577
    Illegal NheI site found at 3747
    Illegal NheI site found at 4789
    Illegal NheI site found at 7501
    Illegal PstI site found at 842
    Illegal PstI site found at 1941
    Illegal PstI site found at 2800
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 226
    Illegal BglII site found at 1211
    Illegal BglII site found at 3235
    Illegal BamHI site found at 3972
    Illegal BamHI site found at 4002
    Illegal BamHI site found at 4088
    Illegal BamHI site found at 4098
    Illegal XhoI site found at 3929
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 842
    Illegal PstI site found at 1941
    Illegal PstI site found at 2800
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 842
    Illegal PstI site found at 1941
    Illegal PstI site found at 2800
    Illegal NgoMIV site found at 3169
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1393
    Illegal BsaI site found at 5170
    Illegal BsaI site found at 8432
    Illegal BsaI.rc site found at 1672
    Illegal BsaI.rc site found at 5449
    Illegal BsaI.rc site found at 6161
    Illegal BsaI.rc site found at 8711
    Illegal SapI site found at 1857
    Illegal SapI site found at 3110
    Illegal SapI site found at 4459
    Illegal SapI site found at 5688
    Illegal SapI.rc site found at 3095
    Illegal SapI.rc site found at 3144
    Illegal SapI.rc site found at 4784
    Illegal SapI.rc site found at 5722


Design Notes

To make compatible to our assembly, CAMV 35S, mCherry and Tnos are amplified by overhang primers and scars of pCnAFP, attB, attP, and phiC31 for golden gate assembly is replaced to ours.



Source

CAMV 35S: BBa_J428074 from distribution 2022 attB:BBa_K2460010 attP:BBa_K2460011 phiC31: BBa_K1742004 mCherry: BBa_J428079 from distribution 2022 Tnos: BBa_J428082 from distribution 2022 pCnAFP: BBa_K4310004 eGFP: BBa_K431006 Kozak: BBa_K4310003

References