Translational_Unit

Part:BBa_K4310014:Design

Designed by: Seungchan Oh   Group: iGEM22_UIncheon   (2022-10-11)


quorum-sensing induced expression of eGFP in plant (1)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 12
    Illegal PstI site found at 938
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 12
    Illegal NheI site found at 1894
    Illegal NheI site found at 3244
    Illegal PstI site found at 938
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 12
    Illegal BglII site found at 30
    Illegal BglII site found at 58
    Illegal BglII site found at 86
    Illegal BglII site found at 114
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 12
    Illegal PstI site found at 938
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 12
    Illegal PstI site found at 938
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1167
    Illegal BsaI site found at 3460
    Illegal BsaI.rc site found at 188
    Illegal BsaI.rc site found at 1446
    Illegal BsaI.rc site found at 3739
    Illegal SapI site found at 202
    Illegal SapI site found at 251
    Illegal SapI site found at 2273
    Illegal SapI site found at 2322
    Illegal SapI.rc site found at 236
    Illegal SapI.rc site found at 983
    Illegal SapI.rc site found at 2307
    Illegal SapI.rc site found at 3276


Design Notes

To make compatible to our assembly, Tnos are amplified by overhang primers and restriction sites of pHSP70A/pRbcS2 for golden gate assembly is replaced to ours.


Source

Tra(4X)::m35S: BBa_K4310000 Kozak: BBa_K4310003 eGFP: BBa_K4310006 Tnos: BBa_J428082 from distribution 2022 pHSP70A/pRbcS2: BBa_K2516001 VP16::TraR: BBa_K4310001

References