Coding
Part:BBa_K4309000:Design
Designed by: Yanqiu Tian Group: iGEM22_NPU-CHINA (2022-09-29)
Laccase(Bacillus)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1207
Illegal AgeI site found at 1348 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1275
Illegal BsaI.rc site found at 1509
Illegal SapI.rc site found at 286
Design Notes
The laccase gene derived from Bacillus subtilis was successfully expressed in Escherichia coli and the degradation efficiency was verified. These works are of great significance to our detailed design of the sequence.
Source
From Bacillus.