Primer

Part:BBa_K4308001:Design

Designed by: Jun Zhang   Group: iGEM22_CPU_CHINA   (2022-09-14)


Reverse Primer for RPA of LINC00857


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to make full use of the detection speed and sensitivity of RAA, the length of the primer should be designed at 30-35 bp, and the length of amplified fragment should be 100-200 bp so that we could obtain fast reaction speed. Also remember, the more pairs of primers screened, the higher the detection accuracy.


Source

Sangon Biotech

References