Composite

Part:BBa_K4307037:Design

Designed by: Chi Zhang   Group: iGEM22_Tsinghua   (2022-10-13)


J32100-AffiPmrB-PmrA-PmrC-T7 core-T3 sigma-pT3-EGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 896
    Illegal BamHI site found at 2161
    Illegal BamHI site found at 2331
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5837


Design Notes

waiting


Source

E.coli

References