![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4307006:Design
Designed by: Chi Zhang Group: iGEM22_Tsinghua (2022-09-30)
attB-promoter-attP & Ser integrase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 361
Illegal PstI site found at 667 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 361
Illegal NheI site found at 350
Illegal PstI site found at 667 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 361
Illegal BglII site found at 525
Illegal BamHI site found at 289
Illegal BamHI site found at 549
Illegal BamHI site found at 927 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 361
Illegal PstI site found at 667 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 361
Illegal PstI site found at 667
Illegal NgoMIV site found at 4
Illegal NgoMIV site found at 1266
Illegal AgeI site found at 124 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 24
Illegal BsaI.rc site found at 1933
Illegal SapI site found at 106
Design Notes
We successfully expressed the integrase from bacteriophage in bacteria, and the results of the fluorescence assay showed that integrase could have obvious inversion effect, but the action time was a little bit long.
Source
E.coli