Device

Part:BBa_K4298007:Design

Designed by: Buyao Li   Group: iGEM22_Peking   (2022-09-29)


Integration Lxr-α-section-EPI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1390
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1694
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 713


Design Notes

The EPI expression Cassette was synthesised. The URA3 5' Homology, URA3 3' Homology and leu2 were from pLeu2.


Source

Assembly by K4298003; K4298006; K1430001; K4298004.