Composite

Part:BBa_K4297063

Designed by: Yang Gu   Group: iGEM22_NJXDF-CHN   (2022-10-10)


pYLXP'-ADH9

The plasmid pYLXP' containing the expressiom cassette of alcohol dehydrogenase ADH9, which can catalyze the reaction of phenylacetaldehyde to 2-PE.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1293
    Illegal NotI site found at 1448
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 249
    Illegal XhoI site found at 4316
    Illegal XhoI site found at 4349
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5081
    Illegal AgeI site found at 950
    Illegal AgeI site found at 6300
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1284
    Illegal BsaI site found at 4596
    Illegal BsaI site found at 6184
    Illegal BsaI.rc site found at 255
    Illegal BsaI.rc site found at 4582
    Illegal BsaI.rc site found at 4608
    Illegal BsaI.rc site found at 5018
    Illegal BsaI.rc site found at 6619
    Illegal SapI site found at 5463


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