Composite

Part:BBa_K4297034

Designed by: Yang Gu   Group: iGEM22_NJXDF-CHN   (2022-10-10)


pYLXP'-ADH1

The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase ADH1, which can catalyze the reaction of phenylacetaldehyde to 2-PE.The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR8, which can catalyze the reaction of phenylacetaldehyde to 2-PE. The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR2, which can catalyze the reaction of phenylacetaldehyde to 2-PE. Next, we transformed plasmids into Y. lipolytica and perform the whole-cell conversion of phenylacetaldehyde. The whole-cell biocatalytic conversion of 1 g/L phenylacetaldehyde by aforementioned strains gave 2-PE titers of 509.34 mg/L.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1293
    Illegal NotI site found at 1448
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 249
    Illegal XhoI site found at 4316
    Illegal XhoI site found at 4349
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5072
    Illegal NgoMIV site found at 5624
    Illegal AgeI site found at 950
    Illegal AgeI site found at 4585
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1284
    Illegal BsaI site found at 4588
    Illegal BsaI site found at 4599
    Illegal BsaI.rc site found at 255
    Illegal BsaI.rc site found at 4582
    Illegal BsaI.rc site found at 4646
    Illegal BsaI.rc site found at 5662
    Illegal BsaI.rc site found at 5673


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