Regulatory
Part:BBa_K4296000
Designed by: Hannah Illing Group: iGEM22_UBC_Vancouver (2022-09-08)
TaHsp70d promoter
A heat-inducible promoter belonging to the heat shock protein family (Xue et al., 2014). Heat shock factors contribute greatly to heat adapation in their eukaryotic hosts. They are transcription factors with domains enabling higher affinity binding to DNA and recruit other regulatory transcription machinery. TaHsp70 is an endogenous wheat promoter with active mRNA expression in response to higher than ambient temperatures. The promoter has optimum activity after 1.5 hours of being induced at 36 degrees Celsius in wheat leaf cells. By engineering biobricks under the control of this temperature regulated promoter, downstream gene expression can be controlled in a heat titratable manner.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 98
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 98
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 119
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 98
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 98
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 296
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Categories
Parameters
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