Plasmid_Backbone

Part:BBa_K4289006

Designed by: Cai Rui   Group: iGEM22_Nanjing_HS   (2022-08-15)


pQE-30


pQE30-backbone is one of the E. coli expression vectors, which uses a T5 promoter to regulate the expression of exogenous genes that could be induced by IPTG. We inserted the hGK2 DNA fragment into the BamHI and SalI sites, and the 6×His tag is fused at the N-terminal of the protein and is used for protein purification. The vector is a low-copy-number plasmid. When expressed in the prokaryotic system, the Amp+ resistance can be used to screen the right colony. This plasmid backbone can be used to express different proteins in the future.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


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