Regulatory

Part:BBa_K4286408:Design

Designed by: Bingru Feng   Group: iGEM22_SZU-China   (2022-09-28)


cbh1 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1006
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Pcdh1-cdh1ls-mazEF-Tcdh1 circuit was integrated into pCAMBIA1302 vector by homologous recombination. We transformed the recombinant plasmid into Trichoderma atroviride by Agrobacterium-mediated transformation in order to achieve condition-triggered suicide of T. atroviride ultimately.


Source

The sequence of cbh1 promoter belongs to Trichoderma koningii cbh1 gene for 1,4-beta-D-glucan-cellobiohydrolase (GenBank: X69976.1), which comes from genomic sequence of Trichoderma koningii.


References

[1]Kluge J, Terfehr D, Kück U. Inducible promoters and functional genomic approaches for the genetic engineering of filamentous fungi. Appl Microbiol Biotechnol. 2018 Aug;102(15):6357-6372. doi: 10.1007/s00253-018-9115-1.

[2]Wey TT, Hseu TH, Huang L. Molecular cloning and sequence analysis of the cellobiohydrolase I gene from Trichoderma koningii G-39. Curr Microbiol. 1994 Jan;28(1):31-9. doi: 10.1007/BF01575983.

[3]Madhavan A, Sukumaran RK. Promoter and signal sequence from filamentous fungus can drive recombinant protein production in the yeast Kluyveromyces lactis. Bioresour Technol. 2014 Aug;165:302-8. doi: 10.1016/j.biortech.2014.03.002. Epub 2014 Mar 12.

[4]Henrique-Silva F, el-Gogary S, Carle-Urioste JC, Matheucci E Jr, Crivellaro O, el-Dorry H. Two regulatory regions controlling basal and cellulose-induced expression of the gene encoding cellobiohydrolase I of Trichoderma reesei are adjacent to its TATA box. Biochem Biophys Res Commun. 1996 Nov 12;228(2):229-37. doi: 10.1006/bbrc.1996.1646. PMID: 8920899.