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Part:BBa_K4286406:Design

Designed by: Bingru Feng   Group: iGEM22_SZU-China   (2022-09-02)


R.solani AG-3 ITS (ITS1 & 5.8SrRNA) sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 322
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 322
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 322
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 322
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 322
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We take advantage of this conservative sequence of R.solani for the detection of rice sheath blight. Our LAMP-LFD system is able to reflect the existance of R.solani in infected rice.


Source

It comes from genomic sequence of Rhizoctonia solani AG-3.

References