Composite

Part:BBa_K4286207:Design

Designed by: Bingru Feng   Group: iGEM22_SZU-China   (2022-10-09)


PCaMV-snakin1-6xHis-TNOS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 914
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 66


Design Notes

Evidence shows that the antimicrobial peptide Snakin-1 can effectively inhibit Rhizoctonia solani. In this project, we hope to express Snakin-1 constitutively in Trichoderma atroviride to improve its lethality against R. solani.


Source

Snakin-1 comes form Solanum bulbocastanum.


References

[1]Molla KA, Karmakar S, Molla J, Bajaj P, Varshney RK, Datta SK, Datta K. Understanding sheath blight resistance in rice: the road behind and the road ahead. Plant Biotechnol J. 2020 Apr;18(4):895-915. doi: 10.1111/pbi.13312. Epub 2020 Jan 29.

[2]Almasia NI, Bazzini AA, Hopp HE, Vazquez-Rovere C. Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants. Mol Plant Pathol. 2008 May;9(3):329-38. doi: 10.1111/j.1364-3703.2008.00469.x.

[3]Kuddus MR, Yamano M, Rumi F, Kikukawa T, Demura M, Aizawa T. Enhanced expression of cysteine-rich antimicrobial peptide snakin-1 in Escherichia coli using an aggregation-prone protein coexpression system. Biotechnol Prog. 2017 Nov;33(6):1520-1528. doi: 10.1002/btpr.2508. Epub 2017 Jun 12.