Composite

Part:BBa_K4286206:Design

Designed by: Bingru Feng   Group: iGEM22_SZU-China   (2022-10-09)


PCaMV-epl1-6xHis-TNOS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 66


Design Notes

EPL1 is a kind of hydrophobic protein located on the surface of fungi and has been proven to induce defence responses in plants. In the project, we plan to enhance the hydrophobicity of Trichoderma atroviride mycelium by overexpressing EPL1 to enable it to float on the water surface, and to enhance the defense response of rice to help it resist Rhizoctonia solani.


Source

EPL1 comes from Trichoderma atroviride IMI 206040.

References