Composite
Part:BBa_K4286203:Design
Designed by: Bingru Feng Group: iGEM22_SZU-China (2022-10-09)
PCaMV-prb1-6xHis-TNOS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1946
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1046
Illegal NgoMIV site found at 1091
Illegal NgoMIV site found at 1385
Illegal NgoMIV site found at 1499
Illegal AgeI site found at 2015 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 66
Illegal BsaI site found at 1232
Illegal BsaI.rc site found at 1109
Design Notes
The protease PRB1 has been proven to be an effective biocontrol agent against a variety of plant fungal pathogens. In our design, PRB1 will be overexpressed in Trichoderma atroviride to enhance its inhibitory effect on Rhizoctonia solani.
Source
PRB1 comes from Trichoderma atroviride IMI 206040.