Primer

Part:BBa_K4286001:Design

Designed by: Bingru Feng   Group: iGEM22_SZU-China   (2022-09-02)


RSAGF3, the primer of R.solani AG-1&AG-3 ITS (ITS1 & 5.8SrRNA) sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

With this primer and other primers, LAMP reaction can rapidly amplify ITS sequence of R.solani. The successfully amplified product can be detected by LAMP-LFD detection strip, indicating that the corresponding sample contains the pathogen, R. solani AG-1, thus achieving a rapid, convenient and sensitive detection of rice sheath blight.


Source

The primer is chemically synthesized, which complements the bases of R.solani ITS sequence.

References