Plasmid_Backbone
Part:BBa_K4284021:Design
Designed by: Zhang Xinchi Group: iGEM22_PINGHE (2022-08-11)
pACYC-acs-backbone
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 83
Illegal XbaI site found at 2564
Illegal PstI site found at 102 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 83
Illegal NheI site found at 1723
Illegal PstI site found at 102
Illegal NotI site found at 120 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 83
Illegal BglII site found at 276
Illegal BamHI site found at 77
Illegal XhoI site found at 325 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 83
Illegal XbaI site found at 2564
Illegal PstI site found at 102 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 83
Illegal XbaI site found at 2564
Illegal PstI site found at 102
Illegal NgoMIV site found at 295
Illegal AgeI site found at 537
Illegal AgeI site found at 1809
Illegal AgeI site found at 2132 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Design Notes
NO
Source
NO