Plasmid_Backbone

Part:BBa_K4284004:Design

Designed by: Zhang Xinchi   Group: iGEM22_PINGHE   (2022-08-11)


pCDFD-bktB-ydil-backbone


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1241
    Illegal XbaI site found at 3355
    Illegal PstI site found at 329
    Illegal PstI site found at 968
    Illegal PstI site found at 1260
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1241
    Illegal NheI site found at 2555
    Illegal PstI site found at 329
    Illegal PstI site found at 968
    Illegal PstI site found at 1260
    Illegal NotI site found at 1278
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1241
    Illegal BamHI site found at 1235
    Illegal XhoI site found at 1343
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1241
    Illegal XbaI site found at 3355
    Illegal PstI site found at 329
    Illegal PstI site found at 968
    Illegal PstI site found at 1260
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1241
    Illegal XbaI site found at 3355
    Illegal PstI site found at 329
    Illegal PstI site found at 968
    Illegal PstI site found at 1260
    Illegal NgoMIV site found at 1808
    Illegal AgeI site found at 1112
    Illegal AgeI site found at 1555
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

NO


Source

NO

References