Part:BBa_K4283012

UV controlled OMV release

OMV released was controlled using the SulAp promoter (BBa_K518010), an optogenetic regulatory element that was previously used by our team in 2020.

Figure 1

Design of light control OMV release

Figure 2

UV control OMV producing vector pSB1C3_sulAp_ClyA-Adpgk-eGFP

After transforming the vector into E.coli Top10 the bacteria were exposed to UV radiation (15mW/CM2) for 1min and tested for change of the florescence.

Figure 3

Change in Florescence of bacteria culture

The florescence of bacteria culture increases significantly after exposure to UV. It was decided to perform further testing through western blot.

Figure 4

Western blot image of bacteria after UV radiation

More information on:Engineering Success | SZ-SHD - iGEM 2022