Part:BBa_K4279006
pUC57-W1 lipase
pUC57-W1 lipase
Profile
Name: pUC57 W1-lipase
Base Pairs: 3511 bp
Origin:E.coli, synthetic
Properties: Plasmid carrying a lipase gene, which can be used as template for amplification of W1-lipase.
Usage and Biology
BBa_K4279006 is the derivative plasmid carrying gene for W1-lipase from pUC57. Lipase is a primary lipase critical for triacylglyceride digestion in humans and is considered a promising target for the treatment of obesity [1]. Triacylglycerol lipase is the primary lipase secreted by the pancreas, and is responsible for breaking down dietary lipids into unesterified fatty acids (FAs) and monoglycerides (MGs). Medically, lipases are targets for therapeutic intervention in the treatment of obesity. The focus of applied research with lipases has been to exploit the unusual properties of lipolytic systems for the production of chiral pharmaceuticals, improved detergents, and designer fats [2]. Obesity is a medical condition in which excess body fat accumulates to the extent that it may have a negative effect on health, leading to reduced life expectancy and/or increased health problems. Diverse approaches to the prevention and treatment of obesity have been reported [3-5]. W1-lipase is a lipase amplified from Lactiplantibacillus Plantarum (LP1406), which is a gram-positive lactic acid bacteria species and exhibits ecological and metabolic adaptability and is capable of inhabiting a range of ecological niches including fermented foods, meats, plants, and the mammalian gastrointestinal tract. The W1-lipase is made up of 265 aa [6].
Construct design
The gene for W1-lipase was inserted into pUC57 (Figure 1).
The profiles of every basic part are as follows:
BBa_K4279001
Name: W1-lipase
Base Pairs: 1854 bp
Origin: Lactiplantibacillus Plantarum, genome
Properties: a lipase for triacylglyceride digestion.
Usage and Biology
BBa_K4279001 is the coding sequence of W1-lipase, which is amplified from Lactiplantibacillus Plantarum (LP1406) and exhibits ecological and metabolic adaptability and is capable of inhabiting a range of ecological niches including fermented foods, meats, plants, and the mammalian gastrointestinal tract [1].
BBa_K4279004
Name: pUC57-backbone
Base Pairs: 2710 bp
Origin: E.coli, synthtic
Properties: cloning vector.
Usage and Biology
BBa_K4279004 is the cloning vector (Figure 2) [2] carrying the beta-galactosidase (LacZ) gene [3], lac repressor [4], and bla gene encoding beta-lactamase conerring resistance to ampicilin [5].
Reference
[1] S.P.S. Chundawat, V. Balan, L. Da costa Sousa, B.E. Dale, Thermochemical pretreatment of lignocellulosic biomass. Bioalcohol Production. 2010, 24-72.
[2] Mohammadi N, Bandehpour M, Sotoodehnejadnematalahi F, Kazemi B. Prokaryotic expression, evaluation, and prediction of the structure and function of the ecarin metalloproteinase domain. Proteins. 2022;90(3):802-809.
[3] Trimborn L, Hoecker U, Ponnu J. A Simple Quantitative Assay for Measuring β-Galactosidase Activity Using X-Gal in Yeast-Based Interaction Analyses. Curr Protoc. 2022 2(5):e421
[4] Effendi SSW, Ng IS. Reprogramming T7RNA Polymerase in Escherichia coli Nissle 1917 under Specific Lac Operon for Efficient p-Coumaric Acid Production. ACS Synth Biol. 2022 Sep 10. doi: 10.1021/acssynbio.2c00363. Epub ahead of print. PMID: 36087056.
[5] Habibzadeh N, Peeri Doghaheh H, Manouchehri Far M, Alimohammadi Asl H, Iranpour S, Arzanlou M. Fecal Carriage of Extended-Spectrum β-Lactamases and pAmpC Producing Enterobacterales in an Iranian Community: Prevalence, Risk Factors, Molecular Epidemiology, and Antibiotic Resistance. Microb Drug Resist. 2022;28(9):921-934. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3493
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 435
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2762
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1790
Illegal SapI site found at 707
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