Plasmid

Part:BBa_K4278722:Design

Designed by: Shi Lifang   Group: iGEM22_Shanghai_Metropolis   (2022-09-20)


pYES2-gRNA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1026
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3059
    Illegal SpeI site found at 1026
    Illegal NotI site found at 1064
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1026
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1026
    Illegal AgeI site found at 335
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1153


Design Notes

no


Source

no

References