Plasmid

Part:BBa_K4278721:Design

Designed by: Shi Lifang   Group: iGEM22_Shanghai_Metropolis   (2022-09-20)


pHCas9-Nours


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 177
    Illegal BglII site found at 6605
    Illegal BglII site found at 7542
    Illegal XhoI site found at 9898
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 942
    Illegal NgoMIV site found at 9094
    Illegal AgeI site found at 660
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 6088
    Illegal SapI site found at 2190
    Illegal SapI.rc site found at 3527
    Illegal SapI.rc site found at 5631


Design Notes

no


Source

no

References