Regulatory

Part:BBa_K4275051:Design

Designed by: Zheng Xiaoyou   Group: iGEM22_GreatBay_SCIE   (2022-10-12)


Kluyveromyces marxianus LAC4 Promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 331
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 331
    Illegal NheI site found at 89
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 331
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 331
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 331
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. The GAL4 Promoter from K.marxianus is assembled into the secretory protein expression vector via goldengate assembly, with two BsaI cleavage sites and two unique overhangs.

2. To construct a K.marxianus secretory expression vector, the plasmid backbone, pLAC4 promoter, MFalpha, mTurquiose and tTDH1 terminator are firstly assembled as the vector for later assembly operations.

Source

Kluyveromyces marxianus CBS397

References

1. Rajkumar, Arun S. et al. "Biological Parts For Kluyveromyces Marxianus Synthetic Biology". Frontiers In Bioengineering And Biotechnology, vol 7, 2019. Frontiers Media SA, https://doi.org/10.3389/fbioe.2019.00097.