Device

Part:BBa_K4275001:Design

Designed by: Zihuan Zhang   Group: iGEM22_GreatBay_SCIE   (2022-09-29)


C1.Ori


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. The C1 origin of replication from K.marxianus is assembled into the secretory protein expression vector via goldengate assembly, with two BsaI cleavage sites and two unique overhangs (gagt&ggca).

2. To construct a K.marxianus secretory expression vector, the plasmid backbone, pLAC4 promoter, MFalpha, mTurquiose and tTDH1 terminator are firstly assembled as the vector for later assembly operations.


Source

Kluyveromyces Marxianus DMKU3-1042

References

1.Rajkumar, Arun S. et al. "Biological Parts For Kluyveromyces Marxianus Synthetic Biology". Frontiers In Bioengineering And Biotechnology, vol 7, 2019. Frontiers Media SA, https://doi.org/10.3389/fbioe.2019.00097.