DNA

Part:BBa_K4274007:Design

Designed by: Jiawen Chen   Group: iGEM22_KEYSTONE   (2022-09-30)


patB_target(gRNA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This year, we are using ClSS_S533A to construct composite parts ptac-RiboJ-B0034-ClSS_S533A-B0034-ERG20-B0015 (part: BBa_K4274020), ptac-RiboJ-B0034-ClSS_S533A-B0034-ERG20_F96W-B0015 (part: BBa_K4274021) and ptac-RiboJ-B0034-ClSS_S533A-FL-ERG20_F96W-B0015 (part: BBa_K4274023). This will allow engineering E.coli DH5a (tnaA-) to heterologously express ClSS_S533A. Other teams can utilize this part for E. coli alpha-santalene production.


Source

Bacillus subtilis

References