Other
Part:BBa_K4274006:Design
Designed by: Jiawen Chen Group: iGEM22_KEYSTONE (2022-09-30)
PvanP*
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For the constitutive expression of sgRNA (patB gene and sfp gene) in pJOE8999, the semi-synthetic promoter PvanP* was used [1]. Two fragments for the deletion of the rnc gene were inserted, one with fabG and acpA homology and the other with smc gene.
Source
Corynebacterium glutamicum