Coding

Part:BBa_K4274002:Design

Designed by: Jiawen Chen   Group: iGEM22_KEYSTONE   (2022-09-30)


ERG20_F96W


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 899
    Illegal BglII site found at 960
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 615
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Erg20(Part Number:BBa_K849001), a bifunctional enzyme, catalyzes the sequential formation of GPP and FPP. The residues F96 of Erg20 is essential for FPP synthesis[1]. In previous research, it was noted that Erg20 mutant F96W was conducive to FPP generation in E. coli[2].


Source

S. cerevisiae

References