Composite

Part:BBa_K4271001:Design

Designed by: Ethan Ho   Group: iGEM22_Wego_Taipei   (2022-08-31)


T7 Promoter + Lac operator + RBS + OPH + T7 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1259
    Illegal NotI site found at 1219
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1228
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 330
    Illegal AgeI site found at 150
    Illegal AgeI site found at 435
    Illegal AgeI site found at 570
    Illegal AgeI site found at 633
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

DNA sequence of the OPH is optimized for the protein expression on E. coli.

Source

pET22b vector, T7 bacteriophage, OPH from phosphotriesterase [Agrobacterium tumefaciens]

References