Regulatory

Part:BBa_K4263010:Design

Designed by: Hanlin Li   Group: iGEM22_SCUT-China   (2022-09-26)


SV40-VP16-EL222


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

when n = 5,the blue-light-induced promoter had the best activity and thelowest leakage while (C120)-minP was not so satisfactory(Wang et al.,2022).So we created PminCYC-(C120)5.


Source

The cofactor used by this optogenetic system is FMN (flavin mononucleotide) EL222 protein originated from Erythrobacter litoralis.

References