PDAS2
Introduction
DAS2 is a promoter derived from dedihydroxy-acetone synthase (DAS) gene[1].
Characterization
In order to test the function of PDAS2, we construct "PDAS2-EGFP-terminator" (Figure 1). If PDAS2 is functional, we can test the fluorescence intensity of EGFP in supernatant samples obtained from the culture of recombinant P.pastoris GS115 strain.
![](https://static.igem.wiki/teams/4263/wiki/parts/image/das2e-min.jpg)
Figure 1 Gene circuit of PDAS2-EGFP-terminator
Our results matched the general expected trend (Figure 2). After fermentation experiment in BMMY medium containing 1% methanol. The fluorescence intensity of the samples of recombinant P.pastoris GS115 containing the EGFP gene gradually increased over time. At the same time, we measured the growth curve of the strains.
![](https://static.igem.wiki/teams/4263/wiki/parts/image/pdas2-min.jpg)
Figure 2 Fluorescence intensity and OD600 absorbance of samples obtained at different time points from the culture of corresponding recombinant P.pastoris GS115 containing EGFP gene.
Reference
[1] Vogl T, Kickenweiz T, Pitzer J, et al. Engineered bidirectional promoters enable rapid multi-gene co-expression optimization[J]. Nat Commun, 2018,9(1):3589.