PACC1
Introduction
ACC1 is the major member of ACCs in mammalian[1].
Characterization
In order to test the function of PACC1, we construct "PACC1-EGFP-terminator" (Figure 1). If PACC1 is functional, we can test the fluorescence intensity of EGFP in supernatant samples obtained from the culture of recombinant P.pastoris GS115 strain.
Figure 1 Gene circuit of PACC1-EGFP-terminator
Our results matched the general expected trend (Figure 2). After fermentation experiment in BMMY medium containing 1% methanol. The fluorescence intensity of the samples of recombinant P.pastoris GS115 containing the EGFP gene gradually increased over time . At the same time, we measured the growth curve of the strains.
Figure 2 Fluorescence intensity and OD600 absorbance of samples obtained at different time points from the culture of corresponding recombinant P.pastoris GS115 containing EGFP gene.
Reference
[1] Wang Y, Yu W, Li S, et al. Acetyl-CoA Carboxylases and Diseases[J]. Front Oncol, 2022,12:836058.