Composite

Part:BBa_K4260222

Designed by: Melchor Andrés Soto Herrera   Group: iGEM22_TecCEM   (2022-10-11)


T7 promoter+LacO+RBS+RecA mini intein ESR1 protein with OmpA, linker and AmilCP+T7 terminator

This part consists of T7 promoter + Lac Operator + RBS + RecA mini intein ESR1 protein with OmpA signal peptide, linker and AmilCP + T7 terminator. It allows the expression of RecA mini intein ESR1 protein when IPTG is added to the used media. After induction with IPTG, several assays can be performed in order to measure EDCs through a change in coloration, using an AmilCP chromoprotein as N and C extein, and ligating after the ESR1 receptor protein binds to an EDC.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3146
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None