Coding

Part:BBa_K4260032:Design

Designed by: Claudia Angélica García Alonso   Group: iGEM22_TecCEM   (2022-10-13)


Iso: Isoeugenol Monooxygenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 696
    Illegal NgoMIV site found at 1304
    Illegal AgeI site found at 520
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 616


Design Notes

Codon optimization (using as base E. coli K12) was performed


Source

This gene is originary from Pseudomonas putida IE27

References