Coding

Part:BBa_K426003

Designed by: Daniela Mehech   Group: iGEM10_Berkeley   (2010-10-26)


tn5

This part encodes a DNA modifying enzyme. Unlike Sleeping Beauty and piggyBac which comes from eukaryotic organisms, Tn5 comes from enterobacterial tranposons.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 591
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 926
    Illegal PstI site found at 591
    Illegal NotI site found at 504
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 591
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 591
    Illegal AgeI site found at 914
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1058
    Illegal BsaI.rc site found at 879
    Illegal SapI site found at 15
    Illegal SapI.rc site found at 327


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Categories
Parameters
None