Composite
Pp_22
Part:BBa_K4260012:Design
Designed by: Claudia Angélica García Alonso Group: iGEM22_TecCEM (2022-10-09)
Isoeugenol Monooxygenase, autoinducible coding sequence: Trp terminator, IemR, promoter, pelB, Iso a
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 451
Illegal XhoI site found at 279
Illegal XhoI site found at 306 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1199
Illegal NgoMIV site found at 1907
Illegal NgoMIV site found at 2515
Illegal AgeI site found at 1731 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1135
Illegal BsaI.rc site found at 1827
Design Notes
Since the coding sequence of IemR was in the antisense chain, the reverse-complementary sequence of the Trp terminator was used, in order to stop the transcription of the IemR.
Source
The transcriptional activator (IemR) and the promoter, come from Pseudomonas nitroreducens Jin1. The Isoeugenol monooxygenase comes from Pseudomonas putida IE27.