Coding

Part:BBa_K4259006:Design

Designed by: Jennifer Adami   Group: iGEM22_Leiden   (2022-10-07)


napA from E. coli


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1873
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1873
    Illegal NotI site found at 2339
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1731
    Illegal BamHI site found at 1080
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1873
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1873
    Illegal NgoMIV site found at 1281
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The plasmid was transformed into E. coli BL21


Source

The plasmid was obtained from the ASKA library

References