Plasmid

Part:BBa_K4258049:Design

Designed by: Jake Schumacher   Group: iGEM22_NDSU   (2022-10-09)


AraC+CDS#19+B0015+DVK


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1365
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1365
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1365
    Illegal BamHI site found at 1218
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1365
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1365
    Illegal NgoMIV site found at 1344
    Illegal AgeI site found at 1053
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1333
    Illegal BsaI.rc site found at 1312
    Illegal BsaI.rc site found at 2393
    Illegal SapI site found at 1035


Design Notes

All parts were designed to be Golden Gate MoClo compatible with Bsa1 sites.


Source

BBa_K4258006 BBa_K4258019 BBa_B0015

References